Journal: Infection and Immunity

Article Title: A Candida albicans Mannoprotein Deprived of Its Mannan Moiety Is Efficiently Taken Up and Processed by Human Dendritic Cells and Induces T-Cell Activation without Stimulating Proinflammatory Cytokine Production

doi: 10.1128/iai.00669-08

Figure Lengend Snippet: FIG. 1. Uptake of rMP65 fluor by different cell types. (A) MN, MDM, DC, PMN, and PBL treated for 30 min at 37°C with rMP65 fluor (5 g/ml) or rCpPLD fluor (5 g/ml) were analyzed by flow cytometry to assess rMP65 and rCpPLD uptake. The rMP65 fluor uptake by different cell types is expressed as the mean fluorescence intensity (MFI). Data are expressed as means SEM for three independent experiments. *, P 0.05; **, P 0.001 (rMP65 fluor-treated cells versus rCpPLD fluor-treated cells). (B) rMP65 fluor, nMP65 fluor, and rCpPLD fluor uptake by DC, tested using confocal microscopy. DC were treated with 25 g/ml of rMP65 fluor, rCpPLD fluor, or nMP65 fluor for 2 h at 37°C. The cells were then fixed and analyzed with a confocal microscope. (C) MDM or DC were stimulated for 30 min at 37°C with different concentrations of rMP65 fluor (0.5, 1, 2.5, 5, 25, and 50 g/ml). The rMP65 fluor uptake by different cell types is expressed as the MFI. Data are expressed as means SEM for three independent experiments. *, P 0.05; **, P 0.001 (rMP65-treated cells versus untreated cells). (D) MDM were pretreated with various concentrations of RGDS or control peptide for 15 min, and then rMP65 fluor (25 g/ml) was added. Data are expressed as means SEM for three independent experiments. *, P 0.05 (RGDS-treated cells versus untreated cells).

Article Snippet: Images were collected on a Nikon C1 laser scanning confocal unit (Nikon D-Eclipse C1) attached to an inverted fluorescence microscope (Nikon Eclipse TE 2000-U).

Techniques: Cytometry, Confocal Microscopy, Microscopy, Control